Optimization of Determination for Total Flavonoids in Euonymus alatus

Abstract:

Objective: To establish a method for determination of the content of total flavonoids in Euonymus alatus. Method: Selecting rutin as a reference substance, single factor tests were adopted to optimize colorimetry methods and conditions, determination of the content of total flavonoids was established and optimized its extraction method. Result: Hydrochloric acid-Mg reaction assay was adopted, rutin had a liner relationship with absorbance in the range of 0.021-0.104 mg (r=0.997), average recovery was 99.424% with RSD of 2.220%. Conclusion: HCl-Mg colorimetry method appeared to be an accurate, reliable and repeatable system for determination of total flavonoids in E. alatus.Total flavonoids in E.alatus should employ reflux extraction method.