Determination of Astragaloside Ⅳ in Shenfu Yiqi Anshen Mixture by HPLC-ELSD

Abstract:

Objective: To establish a HPLC-ELSD method for the determination of astragaloside Ⅳ in Shenfu Yiqi Anshen Mixture. Method: HPLC-ELSD was used to determine astragloside Ⅳ in Shenfuyiqi Anshen Mixture, The separation was performed on Appllo C₁₈ column (4.6 mm×250 mm, 5 μm) with the mixture of acetonitrile-water (36:64) as the mobile phase. The flow rate was 1.0 mL·min^-1, and colume temperature at 30 ℃.The temperature of drift tube was 80 ℃ and zhe carrier flow was 2 L·min^-1.Resuts:The linear ranges of astragaloside Ⅳ was 2.032-12.192 μg (r=0.9999), with the average recovery rate were of 98.42%, RSD was 1.36%. Conclusion: The methods is simple, accurate, reproducible and it can used for the quality control of Shenfu Yiqi Anshen mixture.