Chemical Constituents of Ethyl Acetate Extract from Stenoloma chusanum

Abstract:

Objective: To study the chemical constituents of ethyl acetate extracts from Stenoloma chusanum. Method: Use silica gel column chromato-graphy and Sephadex LH-20 column chromatography to separate and purify the compounds. Structure identification was carried out by nuclear magnetic resonance (NMR) technology. Result: Thirteen compounds were separated and purified from ethyl acetate parts of S. chusanum, and identified as β-sitosterol (1), 4-hydr-oxy-2, 6-dimethoxy-benzoic acid (2), daucosterol (3), apigenin (4), apigenin-7-O-β-D-glucopyranosid (5), 4-hydroxy-3-methoxy-benzoic acid (6), syringic acid (7), protocatechuic aldehyde (8), 2, 5-dihydroxy-benzoic acid (9), protocatechuic acid (10), fraxetin (11), 6-chlorine-apigenin-7-O-β-D-glucopyranosid (12), vitexin (13). Conclusion: Compound 6 and 12 are discovered from Stenoloma for the first time.