Abstract:Objective: To explore the distribution of the total alkaloid in the Fritillaria ussuriensis, to provide the data basis for the ground part of the F. ussuriensis development and utilization. Method: Thin-layer chromatography was used to preliminary determine whether the alkaloid components in F. ussuriensis different growth area were the same, and total alkaloid content of different origin and growth period of Fritillaria ussuriensis aboveground and underground part by acid dye colorimetry. Result: Therewas a difference in F. ussuriensis alkaloid component of different growth area. Total alkaloid contents in cultivated F. ussuriensis ground parts of the vegetative growth, flowering, fruiting period were 2.361, 2.310, 2.193 times more than underground parts. Wild F. ussuriensis aerial parts of vegetative growth, flowering phase were 1.870, 1.496 times more than underground parts. Conclusion: In F. ussuriensis plants' total alkaloid content with some fluctuations, and different growth parts of the alkaloid component there is a difference, but in general the total alkaloid content of F. ussuriensis ground stems and leaves was significantly higher than the underground bulbs, so there is a certain development and utilization value.