Isolation and Identification of Ginsenoside Rg3 from Heat-processed Gocaekmbaw of Zhuang-medicine

Abstract:

Objective: To isolate and identify saponins from Zhuang-medicine Gocaekmbaw (Gynostemma pentaphyllum) after heat-processing. Method: The leaves of gocaekmbaw were steam-heated at 125 ℃ and 0.24 MPa for 3 h, then extracted with 80% ethanol for 3 h. The extracts were isolated with chromatography using HP-20, silica gel and reversed ODS column. The constituents isolated from gocaekmbaw after heat-processing were identified with ¹H-NMR, ¹³C-NMR and LCMS-IT-TOF spectra. Result: Two different configurations of saponins were obtained from the heat-processed Gocaekmbaw. They were identified as 20 (R) -ginsenoside Rg₃ and 20 (S)-ginsenoside Rg₃ and there was no data reported before the present study. Conclusion: Through the method of heat-processing, ginsenoside Rg₃ can be gained from Gocaekmbaw. This may offer a new direction to the preparation of ginsenoside Rg₃.