Nanoparticles in vitro Antioxidant Activity of Polysaccharides from Chinese Yam

Abstract:

Objective: To prepare nanoparticles containing polysaccharides from Chinese Yam by using wet grinding, and opyomize the best prescription by central composite design. Method: To distribute test average particle size and particle size distribution by SALD-2201 laser particle size. Studies on 6 in vitro antioxidant indexes superoxide anion as free radical and hydrogen peroxide, hydroxyl radicals, DPPH, reduction potentials and metal chelating. Result: optimal preparation of Chinese Yam polysaccharides on nanoparticles technology as Liquid is 1:24, ball mill is 36 h, speed time of ball mill is 360 r·min^-1.The size of made from Nano-particle as 1% size is 186 nm, 80.0% size is 575 nm. Conclusion: The antioxidant in vitro experiments confirmed that the yams over nanoparticles in vitro antioxidant activity of polysaccharides from Chinese Yam polysaccharides.