Decomposition Study on Effects of Lianli Tang on Colon and Peripheral Blood Inflammatory Factors in TNBS-induced Rat Model

Abstract:

Objective: To carry out decomposition study on effects of Lianli Tang on colon and peripheral blood inflammatory factors in TNBS-induced rat model. Method: Lianli Tang were divided into three groups, tonify the spleen drugs (Panax Ginseng, Poria cocos, Atractylodes macrocephala koidz, Glycyrrhizae Radix et Rhizoma; symbol as A), Warming the middle and dissipating cold drugs (Zingiberis Rhizoma;symbol as B), clearing heat and drying dampness drugs (Coptidis Rhizoma; symbol as C), as three factors to design the experiment using orthogonal design table L₈ (2⁷), set up 1-7 group and UC group, then add sulfasalazine (SASP) and normal controls group (Control). Sprague Dawley rats were used to duplicate UC model by TNBS/ethanol solution through enema, 1-7 group was administrated respectively by Lianli Tang decompositions through intragastric treatment, which the corresponding doses is 1.02, 1.47, 0.98, 1.24, 0.29, 0.145, 0.087 g·kg^-1, SASP group rats were fed by sulfasalazine (0.4 g·kg^-1). Cytokines of peripheral blood and colon were observed after rats were treated for 14 days. Result: Cyclooxygenase-2 (Cox-2), interleukin-1α ( IL-1α) of UC rats were increased significantly than control (P<0.01), tumor necrosis factor-α (TNF-α) expression were also significantly enhanced, A of Liali Tang significantly affect the effect of inhibiting colon Cox-2 (P<0.05), each factors except the whole formula, have no effect in regulating colonic IL-1α, A×B significantly affected the results (P<0.05) in reducing colonic TNF-α, C, A×C (P<0.01) and B, B×C (P<0.05) in inhibiting colon tissue inhibitor of metall oproteimses 1 (TIMP-1) levels. Serum IL-1α, TNF-α of UC rats decreased apparently, each decomposition factor had no effect on the result;serum TIMP-1 was obviously increased, decomposition C show effect on inhibiting TIMP-1 (P<0.05). Conclusion: Tonify the spleen drugs played a major role in the overall effect of Lianli Tang, Warm the middle and dissipate cold drugs and its compatibility with tonify the spleen drugs also play an important role.