Effect of Ginsenoside on Retinal Müller Cell Cultured in Advanced Glycation End Products Conditions

Abstract:

Objective: To explore the effect of ginsenoside on retinal Müller cell activity under the conditions of advanced glycation end products (AGEs). Method: Modified enzyme-digesting method was used to culture SD rat retinal Müller cells, retinal Müller cells were then cultured in low (terminal concentration:75 mg·L^-1) dose of AGEs conditions and high (terminal concentration:150 mg·L^-1) dose of AGEs conditions, ginsenoside Rg₁, ginsenoside Re, ginsenoside Rb₁ and ginsenoside extractions were added to culture medium respectively. At 24, 48, 72 hours after incubation, the lactate dehydrogenase (LDH) content of extracellular fluid were detected by the enzyme-linked immunosorbent assay (ELISA) to determine Müller cell activity. Result: Compared with normal group the LDH leakage was significantly increased in the low dose of AGEs group at 72 h (P<0.05), whereas there was no difference at 24 h and 48 h;compared with normal group and low dose of AGEs group the LDH leakage were significantly increased in the high dose of AGEs group at each period (P<0.05) ;ginsenoside Rg₁, ginsenoside Re, ginsenoside Rb₁ and ginsenoside extractions could significantly decrease the LDH leakage in the low dose of AGEs group at 48 h and 72 h, and in the high dose of AGEs group at each period (P<0.05). Conclusion: AGEs caused a significant increase of the membrane permeability of retinal Müller cell, decreased the activity of retinal Müller cell, which was related to the time and concentration of intervention;ginsenoside Rg₁, ginsenoside Re, ginsenoside Rb₁ and ginsenoside extractions could decrease the membrane permeability, could stabilize cells' membrane and enhance the activity of retinal Müller cell in AGES conditions, in particular, the effect of ginsenoside extractions is significant.