Effects of Weipixiao on Expression of NF-κB p65 in Gastric Mucosal Epithelial Cells in Rats with Gastric Precancerous Lesions

Abstract:

Objective: To study the effects of Weipixiao, which has functions of fortifying the spleen, dissipating blood stasis, and detoxifying, on gastric precancerous lesions (GPL) and the related molecular mechanisms. Method: SD rats were randomly divided into normal group, model group, Weimeisu group (0.2 g·kg^-1·d^-1), Weipixiao high dose, middle dose and low dose groups (15, 7.5, 3.75 g·kg^-1·d^-1). Except for normal group, the rats in other groups were provided with spontaneous intake of N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) solution, irregular diet and purgative herbs to establish GPL rat model for 18 consecutive weeks. At the beginning of 9th week, the mediation groups were provided with treatment for 10 weeks at corresponding doses mentioned above, and the expression of nuclear factor-kappa B ( NF-κB) p65 in gastric mucosal epithelial cells was observed. Result: Compared with normal group, the scores of NF-κB p65 expression in gastric mucosal epithelial cells of the model group were significantly higher (P<0.01), but decreased in three different dosage of Weipixiao groups (P<0.05), particularly in Weipixiao low dose group (P<0.05). Conclusion: Weipixiao could significantly inhibit the aberrant activation of NF-κB signaling pathway, down-regulating the expression of NF-κB p65 protein.Weipixiao, might probably inhibit the proliferation, invasion and metastasis of atypical cells, as a consequence it can prevent and cure gastric precancerous lesions.