Abstract:Objective: To observe the effect of Huatan Tongluo decoction on aquaporin-4 (AQP-4) protein expression of different brain tissues and S100β of blood serum in rats which use rt-PA thrombolysis after the acute cerebral infarction. Method: SD rats 240 were randomly divided into six groups, sham operation group, model group, rt-PA group, rt-PA Huatan Tongluo of Chinese medicine low dose group (3.6 g·kg-1), rt-PA Huatan Tongluo of Chinese medicine middle dose group (7.2 g·kg-1), rt-PA Huatan Tongluo of Chinese medicine high dose group (14.4 g·kg-1). Using the embolus method prepares the MCAO rat model. rt-PA group and rt-PA Huatan Tongluo group use rt-PA thrombolytic therapy after thrombosis. In 6 hours group the Chinese medicine were given one time, after making mode successfully.In 24 hours group, given two times, after making mode successfully and 2 hours were killed.In 72 hours, given six times, from making mode successfully, two times one day, lasting three days.In 7 day group;given fourteen times, from making mode successfully, two times one day, lasting seven days. Then respectively observe the AQP-4 protein expression in rat cortex and hippocampus by Western blot method, and S100β of blood serum by ELISA method at 6, 24, 72 h, 7 days. Result: Every experimental group in different phases, the AQP-4 protein expression in different brain tissue and S100β of blood serum were highest at 6, 24 h maximum, 72 h, 7 d increased gradually to the level of 6 h. Compared with the model group at the same time the AQP-4 protein expression of middle dose group and high dose group of Chinese traditional medicine were significantly decreased at 24 h or 72 h or 7 d (P<0.05). There was no obvious concentration, but the overall trend showed that Chinese medicine middle dose group and high dose group can obviously decrease the expression of AQP-4.The S100β protein expression didn't show any significant differences in any group, but all have reduced in different degree, and the downward trend is more obvious in Chinese traditional medicine groups. Conclusion: The blood brain barrier structure and function was damaged most seriously at 24 h phase after thrombolysis treatment.Huatan Tongluo combined with rt-PA therapy can reduce AQP-4 expression of different brain tissues and S100β content of serum. So that it can protect the hemorrhagic transformation after thrombolytic treatment of acute cerebral infarction.
