Abstract:Objective: To study the effects of Mongolian pharmaceutical Betel Shisanwei ingredients pill (BSIP) on function of nerve-endocrine-immune in chronic unpredictable stress depression rat and therapeutic mechanism of depression. Method: Sixty male Wistar rats were randomly divided into six groups according to the sugar consumption test and body weight (10 rats in each group) :Normal control group, model group, Fluoxetine group (3.3 mg·kg-1) and high dose, medium dose and low dose group (0.25, 0.5, 1 g·kg-1) of BSIP.Except the normal control, the other groups were treated with the chronic unpredictable mild stress stimulation combined with lonely raising for 28 days.10 mL·kg-1 drugs was given to the rats, once daily, continuously for 28 days. After the experiment blood samples were collected and enzyme-linked immunosorbent (ELISA) was used to detect corticotropin releasing hormone (CRH), adrenocorticotropic hormone (ACTH), corticosterone (CORT), 3, 5, 3'-triiodothyronine (T3), 3, 5, 3', 5'-tetraiodothyronine (T4), thyroid stimulating hormone (TSH), interleukin-2 (IL-2), the content of interleukin-6 (IL-6), and take the thymus, spleen to calculate thymus index, and spleen index. Result: compared with normal control group, model group rats serum CRH, ACTH, CORT, T3, IL-6 levels increased significantly (P<0.01), the TSH levels, spleen index significantly decreased (P<0.01) ;Compared with model group, in the fluoxetine group and BSIP group, the serum CRH, ACTH, CORT, T3, IL-6 content significantly decreased (P<0.01 or P<0.01) ;the TSH levels increased significantly (P<0.05 or P<0.01) ;in the BSIP group, the spleen index increased significan tly (P<0.01), especially in the BSIP high dose group, it was significant. Conclusion: BSIP can adjust the depression model rats hypothalamus-pituitary-adrenal axis and hypothalamus-pituitary-thyroid axis function, and immune function, this could be one of mechanisms.
