Effect of Shangzhongxia General Gout Decoction on Acute Gouty Arthritis and Th17 Cells

Abstract:

Objective: To study anti-inflammatory and analgesic effects of Shangzhongxia generic gout decoction (SGGD) on sodium urate induced acute gouty arthritis (GA). Method: 60 rats were randomly divided into six groups, the control group, the model group, indomethacin group, SGGD high dose group, middle dose group, low dose group (20, 10, 5 g·kg^-1), 10 in each group. SGGD was given once each day for 7 days.1 h after the last administration, according to the classical method Coderre, in addition to the control group, and rats in the remaining five groups were injected ankle injection of sodium urate crystal solution to establish acute gouty arthritis model. The degree of joint swelling were measured the, Flow cytometry was used to detect the content of peripheral blood Th17 cells, ELISA was used to detect serum IL-17, IL-6. Result: SGGD inhibited sodium urate induced ankle pain, decreased the levels of Th17 in peripheral blood, in high dose group, Th17 cells (1.65±0.25) % was significantly lower than that in the model group (3.25±0.96) %. Th17 cell related inflammatory factor IL-17 (0.54±0.09) μg·L^-1 was significantly lower than that in the model group (0.69±0.07) μg·L^-1. Inflammatory IL-6 content was obviously lower than that in the model group (P<0.05). Conclusion: SGGD has better therapeutic effect on sodium urate gouty arthritis, the mechanism may be related to the decreasing Th17 cells and the level of inflammatory factors IL-17, IL-6.