Effects of Processing and Usage on Dissolution of Total Components and Sinapine from Drug Pairs of Raphani Semen and Armeniacae Semen Amarum

Abstract:

Objective: To explore effects of processing and usage on dissolution of water,alcohol soluble total components,sinapine and fatty oil from drug pairs of Raphani Semen and Armeniacae Semen Amarum. Method: The content of sinapine was determined by HPLC,mobile phase was acetonitrile-0.1% phosphoric acid solution(15: 85) and detection wavelength was set at 326 nm;contents of total components in water,alcohol-soluble extract and fatty oil were determined by weight method.Composition and content of fatty acids in Armeniacae Semen Amarum was analyzed by GC-MS with DB-5 quartz capillary column(0.25 mm×30 m),temperature of injection port and gasification room were 230℃ and 270℃,respectively;split ratio was 50: 1,programmed temperature(100-180℃,10℃ · min^-1;180-200℃,4℃ · min^-1;200-220℃,1℃ · min^-1;220-270℃,5℃ · min^-1),MS conditions for EI ion source,70 eV and scanning range of m/z 30-500. Result: Total composition was easy to dissolving out by processing,dissolution of water,alcohol soluble ingredients and sinapine were 9.61%,141.03% and 20.00%,while increase were 0.36-1.85,38.41-62.06,1.40-2.60 times after being crushed,respectively;dissolution between single-decoction and mixed-decoction had no significant differences,dissolution of sinapine reduced 45.16%-68.75% after mixed-decoction. Conclusion: If seeds Chinese medicine not shattered,it do not suitable for extracting by alcohol or wine,crushing can significantly increase solubility of these measured components,mixed-decoction of Raphani Semen and Armeniacae Semen Amarum againsts dissolution of sinapine.Processing in accordance and reasonable application must be combined to ensure curative effect of traditional Chinese medicine.