Grey Correlation Degree Analysis Between Active Ingredients in Barks or Leaves of Eucommiae Cortex and Environmental Factors in Their Habitats

Abstract:

Objective: To find out dominant environmental factors which impact main effective components in barks or leaves of Eucommiae Cortex by gray correlation analysis. Method: HPLC was employed to determine contents of geniposidic acid,aucubin,pinoresinol diglucoside and chlorogenic acid at the same time in barks or leaves of Eucommiae Cortex at 10 years from ten habitats,detection wavelength were 210 nm.Soil physicochemical characteristics were detected,such as total nitrogen,total phosphorus,total potassium and so on,in order to obtain soil factor data,data were analyzed by grey correlation method. Result: There were big differences between barks or leaves of Eucommiae Cortex at the same growth ages in ten habitats.Effects of alkali-hydrolyzable nitrogen,rapidly available potassium,organic matter,the annual average relative humidity,the annual average maximum temperature on contents of four compounds from different tissue were remarkable. Conclusion: Grey correlation analysis finds out dominant environmental factors that impact contents of active ingredients in different tissues of Eucommiae Cortex.It may provide a clue to improve content of secondary metabolites in Eucommiae Cortex by rational application of fertilizer and environmental control,it also provides scientific guidance for choosing suitable areas and analyzing genuineness causes of Eucommiae Cortex.