Optimization of Ultrasonic Extraction Process of Total Triterpenoids from Leaves of Psidium guajava by Response Surface Methodology

Abstract:

Objective: To optimize extraction process of total triterpenoids from leaves of Psidium guajava. Method: Considering yield of total triterpenoids as index,based on single factor experiments,response surface methodology was adopted to optimize extraction process by taking ethanol concentration,liquid-solid ratio and ultrasonic power as factors.UV was employed to determine the content of total triterpenoids at 540 nm. Result: Optimum technological conditions were as follows:extracted twice with 60 times the amount of 70% ethanol at 150 W and 50℃ for 30 min of per time;yield of total triterpenoids was 7.93%,whose deviation was small by comparing with the theoretical value of 7.86%. Conclusion: Response surface analysis used in optimization of total triterpenoids is feasible,this study can provide experimental basis for development and utilization of this plant part.