HPLC Fingerprint of Tibetan Medicine Pterocephali Herba

Abstract:

Objective: The aim of this study was to establish the HPLC fingerprint of Tibetan medicine Pterocephali Herba, and compare the fingerprints of Pterocephali Herba from different places, in order to provide a new method for its quality evaluation. Method: HPLC method was adopted to determine 10 groups of Pterocephali Herba from different places, with Boston Symmetrix(4.6 mm×250 mm,5 μm)chromatographic column. The mobile phase was methanol-0.1 mol· L^-1 ammonium acetate solution(81: 19) at a flow rate of 0.8 mL· min^-1. The detection wavelength was set at 210 nm, column temperature was maintained at 30℃. And the similarities of Pterocephali Herba from different places were compared. Result: The common mode of HPLC fingerprint of Tibetan medicine Pterocephali Herba was established according to the fingerprint similarity evaluation system of Pharmacopoeia Convention (2004A version), and 11 characteristic peaks were marked. The 10 groups of samples had high similarity. Conclusion: This method has good repeatability, precision, and stability, providing scientific basis for quality control of Pterocephali Herba.