Abstract:Objective: To establish TLC fingerprint of active antibacterial fraction of Pilea aquarum. Method: The activity fraction of P. aquarum was confirmed by antibacterial activity assay, and then the TLC fingerprint of 10 batches of P. aquarum from Guizhou was analyzed comparatively. Result: The EtOAc extraction of P. aquarum is antibacterial activity. The TLC chromatograms of 10 bat ches were very similar, which had 8 spots and 8 corresponding peaks, but the intensity of each peak was different. Conclusion: The method is simple and stabilized, and can be used for the quality control of P. aquarum Dunn.