Determination of Cucurbitacin B in Some Parts of Trichosanthes kirilowii by HPLC-UV Method

Abstract:

Objective: To determine the contents of cucurbitacins B in some parts of Trichosanthes kirilowii Maxim. Method The sample solutions were prepared in 50% MeOH by the supersonic extraction. An HPLC-UV method was performed on ODS column (4.6 mm×250 mm, 5 μm) with a mobile phase of methanol-water(70: 30) at a flow rate of 1.0 mL·min^-1, the detection wavelength was at 230 nm, and column temperature at 30℃. Result Cucurbitacins B and E were determined in male root, female root, stem, leaf, young fruit, unripe fruit, ripe fruit, unripe flesh, ripe flesh, seed, male flower, carpopodium, unripe pericarp, and ripe pericarp. The contents of cucurbitacin B were 0-23.13 mg per 100 g sample of each part of T. kirilowii. Cucurbitacin E was not detected in any parts of T. kirilowii. Conclusion: There are different contents of cucurbitacin B in different parts of T. kirilowii. The content of cucurbitacin B was 23.13 mg per 100 g of a stouk root of female T. kirilowii growth for 3 years and lower than 9 mg per 100 g of other samples. The contents of cucurbitacin B were related to some factors such as the growth period and the part of T. kirilowii.