Establishment of the HPLC Fingerprint of Sophora tonkinensis var. polyphylla from Guangxi and Comparison of Concordance with HPLC Fingerprint of S. tonkinensis

Abstract:

Objective: to establish the HLPC fingerprint methods of Sophora tonkinensis var.polyphylla and provide reference for scientific evaluation and quality control of S. tonkinensis var. polyphylla herb. To provide reference data for the Homology study of ophora tonkinensis polyphylla and S.tonkinensis through comparing the fingerprints. Method: An HPLC methods was used. An Agilent Eclipse XDB-C₁₈ (4.6 mm×250 mm, 5 μm) column was adopted, and the mobile phase consisted of acetonitrile and 0.05 mol· L^-1 potassium dihydrogen phosphate solution(adjusted with triethylamine pH 6.4) with a gradient elution. The flow rate was 1.0 mL· min^-1, column temperature was 30℃, and detection wavelength 215 nm. A ‘chromatrographic fingerprint evaluation system 2004A edition’ software was used for data processing. Result: The establishment fingerprint of S. tonkinensis var. polyphylla has 14 common peaks and 7 peaks of them were identified using the reference retention time and UV-DAD detector extracted spectra dual qualitative indicators. Most of the compare similarities of fingerprints of each batch herb were above 0.90, in line with the requirements of fingerprint technology research. Comparing with the fingerprint of S. tonkinensis var.polyphylla and S.tonkinensis, the similarity were above 0.90. Conclusion: The precision, stability and reproducibility of the established method was good. The method could be effectively used for quality control of S. tonkinensis var.polyphylla herb and provide a scientific basis for the development and use of the herb. It could be infer that S. tonkinensis var. polyphylla and S.tonkinensis have similar ingredients and close relationships from the compared study of their fingerprints.