Determination of Catecholamines Excretion in Rats Urinary by HPLC-FD

Abstract:

Objective: To establish an HPLC-FD method for the determination of catecholamines excretion in rats urinary, and hence providing a reference for the pharmacological research of the hyperactivity of Yin deficiency and Huo exuberance. Method: Catecholamines was concentrated by acid alumina adsorption from rats urinary, followed by the analysis on a HiQ-Sil C₁₈V column at 40℃ with the 0.02 mol· L^-1 KH₂PO₄solution as mobile phases. FD excitation wavelength was set a 280 nm and emission wavelength was set a 316 nm, flow rate was 0.5 mL· min^-1 throughout the analysis. Result: Catecholamines could be satisfactorily separated from the interferential components in the sample. The linearity of the calibration curve, precision and repeatability was good with the average recovery reached 65% to 71%. In addition, the sample solution prepared was stable within 16 h(RSD<2%). And, the catecholamines excretion in rats urinary after adjustment raise was stable. Conclusion: The method for sample solution preparation was convenient. Moreover, the HPLC-FD method developed in the study was stable and reliable, and hence can be used to determine the catecholamines excretion in rats urinary.