Abstract:Objective: To explore the influence on matrix metalloproteinase-9 (MMP-9), transforming growth factor-β1 (TGF-β1) and tissue inhibitor of metalloproteinase-1(TIMP-1) liver tissue and nephridial tissue injury in diabetic nephropathy rats by Yishenkang granules. Method: Diabetic nephropathy model was established using unilateral nephrectomy combined with streptozotocin induction. Fourty-three diabetic nephropathy rats were randomly divided into model group, positive group, Yishenkang granules groups (2, 5, 15 g· kg-1). The rats in positive group were ig given 5.2 mg·kg-1 losartan. The rats in Yishenkang granules were given 2, 5, 15 g· kg-1Yishenkang granules, respectively. Another 10 normal rats were assigned to normal group. The rats in normal group and model group were ig given distilled water. All rats were given a 12-week treatment. The blood glucose (GLU), blood urea nitrogen (BUN), creatinine (SCr) and the levels of matrix metalloproteinase-9 (MMP-9), transforming growth factor-β1 (TGF-β1) and tissue inhibitor of metalloproteinase-1(TIMP-1) in rat liver tissue and nephridial tissue were determined. Result: Compared with normal group, the GLU in model group was increased at weeks 6 and 12 (P<0.05). Compared with normal group, the SCr and BUN in model group were increased at weeks 6 and 12 (P<0.05), and the SCr and BUN were decreased in Yishenkang granules of 5 g·kg-1 and 15 g· kg-1 groups after 6- and 12-week treatment (P<0.05). Compared with normal control group, MMP-9 was significantly decreased, TGF-β1 and TIMP-1 were significantly increased in model group (P<0.05). Compared with model group, the changes of MMP-9, TGF-β1 and TIMP-1 in positive control group, Yishenkang granules of 2, 5, 15 g· kg-1groups had significant difference (P<0.05). Conclusion: Yishenkang granules could regulate the expression levels of MMP-9, TGF-β1 and TIMP-1 in liver tissue of diabetic nephropathy rats. It has certain adjustable effect on mechanism of liver injury and could improve renal function at the same time.
