Effects of Qutan Huoxue Decocotion on Expression of Aquaporin 9 in Nonalcoholic Fatty Liver Rats

Abstract:

Objective: To observe the effect of Qutan Huoxue decocotion (QTHXD) on the expression of aquaporin 9 (AQP9) in the nonalcoholic fatty liver rats, and discuss the mechanism. Method: Sixty SD rats were randomly divided into 5 groups,12 each group:the blank control group, model control group,QTHXD low, medium and high dose groups(6,12,16 g· kg^-1),all rats except balnk congtrol group were fed high fat diet for 10 weeks to establish nonalcoholic fatty liver disease(NAFLD) model. After modelingg, the corresponding drugs were ig giventwice a day, for 2 weeks.HE,ELISA and RT-PCR were used to observe the liver pathology changes, total cholesterol(TC),triglycerides(TG) and the AQP9 mRNA expression in liver cells. Result: ① AQP9 expression in liver cells of NAFLD model group compared with blank control group was raised., but decreased in three QTHXD dose 12 g· kg^-1group was lower than the other two groups.②TC and TG in three QTHXD dose groups deacreased, 16 g· kg^-1group was lower than the other two groups.③Hepatic steatosis in the QTHXD different dose groups was reduced compared with NAFLD model group, and hepatic steatosis in 16 g· kg^-1 group reduced more markedly than the other two groups. Conclusion: QTHXD could regulate AQP9 expression of liver cells in NAFLD model rats,reduce TC and TG and improve the degree of hepatic steatosis.