Effect of Ailuo Kechuanning on Expression of p38MAPK, TNF-α and AQP5 Genes in Rats with Chronic Obstructive Pulmonary Disease

Abstract:

Objective: To study the effect of Ailuo Kechuanning on expression of p38MAPK,tumor necrosis factor alpha(TNF-α),and aquaporin 5(AQP5) genes in rats with chronic obstructive pulmonary disease(COPD). Method: Lipopolysaccharide(LPS)smoke was used to COPD rat model,the experimental animals were randomly divided into normal control group,model group,Normal group, model group was given physiological saline(15.52 mL· kg^-1· d^-1) byintragastric, Ailuo Kechuanning group(7.75,15.52,31.04 g· kg^-1· d^-1),ig for 15 days. The expression of p38MAPK,TNF-α and AQP5 genes in the trachea, bronchus and lung tissue were detected by in situ hybridization and immunohistochemical techniques. Result: Compared with the normal group, the expression of mRNA and protein of AQP5 were weakened, but the expression of p38MAPK,TNF-α mRNA and protein were enhanced in model group, the expression of AQP5 protein was negatively correlated with p38MAPK(r=-0.547,P<0.01),TNF-α protein(r=-0.532,P<0.01).Compared Ailuo Kechuanning mediate-dose group with the model group, the expression of AQP5 gene in enhancements, However, the expression of p38MAPK,TNF-α genes was decreased apparently,the expression of AQP5 protein was negatively correlated with p38MAPK(r=-0.542,P<0.01),TNF-α protein(r=-0.541,P<0.01). Conclusion: The mechanism of Ailuo Kechuanning regulating COPD airway mucus hypersecretion may increase AQP5 gene and inhibit p38 MAPK,TNF-α genes expression.

Key words:chronic obstructive pulmonarydisease;p38 mitogen activatein kinase;tumor necrosis factor alpha;aquaporin 5