Effect of Yiqi Huoxue Recipe to MMP-2/TIMP-2 Expression in Radioactive Damaged Lung Tissue

Abstract:

Objective: To study the effect of the Yiqi Huoxue recipe on expression of the matrix metalloproteinase-2(MMP-2)/tissue inhibitor of matrix metalloproteinase(TIMP-2) in radiation-induced lung injury model rats, in order to know the mechanism of this recipe. Method: One hundred and twenty female rats were divided into four groups at random:namely simple exposure group(model group,n=30), radiational Yiqi Huoxue recipe high-dose group and the radiational Yiqi Huoxue recipe low-dose group(n=30),and blank control group(control group,n=30). Six rats were selected randomly from the four groups each time at the 4^th, 6^th, 8 ^th, 12^th, 26^th weekend after first radiation, those rats were executed immediately after intraperitoneal anesthesia, took the right lung tissue of them and saved in right way. Reverse transcription PCR(RT- PCR) was used to detect the dynamic change of mRNA expression of MMP-2, TIMP-2 in each time point.Western-blotting was used to detect the dynamic change of protein expression of MMP-2, TIMP-2 in each time point. Result: The expression of MMP-2 mRNA and protein appeared a first increased and then decline trend from 4^thweekend to 20^th weekend after irradiation, and the peak was in 12^th.The expression of TIMP-2 mRNA and protein appeared a rising tend from 4^thweekend to 20^th weekend after irradiation. The mRNA and protein expression trend is similar to Yiqi Huoxue recipe high-dose group and model group,but they have different change in different time point. In Yiqi Huoxue recipe low-dose group the indicators changed with no statistical significance. Conclusion: High-dose Yiqi Huoxue recipe can adjust the expression of MMP-2/TIMP-2 from the transcription and translation level in each period of radioactive lung injury, making so as to reduce the radioactive lung injury. This may be the molecular biology mechanism of the drug in prevention and control of radioactive lung injury.