Studies on Antitumor Effect and Mechanisms of Sophora tonkinensis and Its Granules

Abstract:

Objective: To observe the inhibitory action of Sophora tonkinensis and its granules on hepatoma H22 ascitic tumor,S180 solid tumor and investigate their mechanisms. Method: Using cyclophosphamide(CTX)as a positive control to compare with Transplantated hepatoma H22 ascitic tumor,S180 solid tumor of mice in vitro. Use the cyclophosphamide(CTX) as positive control, the mice that successful tumor types were were randomly divided into nine groups, which are the normal control group, model group, S.tonkinensis high, medium and low dose group (raw medicine 7.0,3.5,1.75 g· kg^-1), S. tonkinensis granules high, medium and low dose group (raw medicine 7.0,3.5,1.75 g· kg^-1), positive control group (CTX,0.02 g· kg^-1). Two days after grouping, give the positive group ip CTX. Other groups give appropriate medicine once a day, for 11 days. The volumes are 20 mL· kg^-1. Observe the effect of S.tonkinensis and its granules on the weight of hepatoma H22 ascitic tumor and S180 solid tumor, inhibition rate and the organ index. The content of tumour necrosis factor-α(TNF-α),interferon-γ(INF-γ),interleukin-2(IL-2)in mice serum were determined by using radioimmunoassay. Result: The growth of hepatoma H22 ascitic tumor and S180 solid tumor was significantly inhibited by both S.tonkinensis and its granules after a treatment from day 1 to day 10 by ig besides,the level of TNF-α(P<0.05),INF-γ(P<0.05),IL-2(P<0.01) in mice serum was significantly improved. Conclusion: The mechanisms of S.tonkinensis and its granules on hepatoma H22 ascitic tumor and S180 solid tumor may be related to repressing the expression of cytokines.