Effects of Jiawei Duhuo Jisheng Tang on Expression Levels of Inflammatory Factors in Knee Joint Synovial and Serum of Type Ⅱ Collagen-induced Arthritis in Rats

Abstract:

Objective: To observe effects of Jiawei Duhuo Jisheng Tang on mRNA transcription expression levels of interleukin-17(IL-17),tumor necrosis factor-α(TNF-α),interleukin-6(IL-6) in knee joint synovial and protein expression in serum of type Ⅱ collagen-induced arthritis in rats. Method: A rat model of collagen induced arthritis(CIA) was developed with male SPF sprague-dawley rats,thirty CIA rats were divided randomly into 5 groups,the model group were given distilled water(ig,10 mL· kg^-1),the leflunomide group(ig,10 mL· kg^-1),low,medium and high dosage groups of Jiawei Duhuo Jisheng Tang(ig,11,22,44 g· kg^-1),6 rats each group.Rats in the normal group were given the same dosage of distilled water,all rats received oral administration of corresponding medicines once daily for 12 weeks.Levels of interleukin-6(IL-6),interleukin-17(IL-17) and tumor necrosis factor-α(TNF-α) in serum were examined by ELISA,mRNA transcription expression levels of IL-6,IL-17,TNF-α were detected by RT-PCR.Statistical methods was employed by variance analysis. Result: Compared with the normal group,mRNA and protein expression of IL-6,IL-17,TNF-α increased significantly in the model group. Compared with the model group,mRNA and protein of IL-6,TNF-α in treatment groups was down-regulated,protein expression of IL-17 of Jiawei Duhuo Jisheng Tang decreased significantly and mRNA expression of IL-17 was no significant differences. Conclusion: Jiawei Duhuo Jisheng Tang shows a certain therapeutic effect on CIA rats,its anti-inflammatory action mechanism may achieve by reducing expression of IL-6,IL-17 and TNF-α.