Intestinal Absorption Investigation of Scutellarin Ethyl Ester by in situ Single Pass Intestinal Perfusion Model

Abstract:

Objective: To investigate absorption mechanism of scutellarin ethyl ester (DZY-02). Method: In situ single pass intestinal perfusion model was established,HPLC was adopted to study absorptions of three dose groups of DZY-02 in different segments,effects of different protein inhibitors on absorption parameters of DZY-02 in different intestinal segments were investigated. Result: Absorption rate constant and effective permeability coefficient (P_eff) were no significantly differences in different segments of rat intestine or concentration of DZY-02, P_eff was more than 0.2×10^-4 cm·s^-1.Protein inhibitors had no obvious effects on intestinal absorption of DZY-02. Conclusion: DZY-02 is a high-permeability candidate drug and can be absorbed in whole intestinal segments,transport mechanism of DZY-02 in vivo is similar to passive diffusion.DZY-02 may be not a substrate of P-glycoprotein and breast cancer resistance protein.