Optimization of Extraction Process of Volatile Oil from Jingfang Zhiyang Granules by Box-Behnken Design Response Surface Methodology

Abstract:

Objective: To optimize extraction process of volatile oil from Jingfang Zhiyang granules. Method: HPLC was employed to determine the content of pulegone with mobile phase of methanol-water and detection wavelength at 252 nm.Taking yield of volatile oil and the content of pulegone as indexes,Box-Behnken design-response surface methodology was adopted to optimize extraction process of volatile oil with soaking time,extraction time and solid-to-liquid ratio as factors. Result: Optimum extraction process was as follows:soaked 2 h and extracted 6.5 h with 9 times the amount of water.Yield of volatile oil was 0.69 mL with the content of pulegone was 38.17 g·L^-1,these values were similar to their predictive values. Conclusion: This optimized extraction process is reasonable and feasible,which can effectively retain volatile ingredients in Jingfang Zhiyang granules.