Analysis Method of Alpha Asarone in Situ Gel in Vitro

Abstract:

Objective: Set up the analysis method of alpha asarone in situ gel and the analysis method of it's in vitro release. Method: HPLC instrument with SHIM-PACK VP-ODS column(4.6 mm×250 mm,5 μm)was used.The mobile phase consisted of methanol-water (75 : 25),The wavelength for detection was 258 nm. And using pH 7.4 slightly PBS as release liquid. Result: The liner range of alpha asarone was 10.4-104 mg·L^-1(r=1).The average recovery was 100.6%,RSD was 2.19% in the determination of experiment.The liner range of alpha asarone was 2.06-82.4 mg·L^-1(r=0.999 9).The average recovery was 95.83%,RSD was 2.5% in the in vitro release experiment. Conclusion: The method is exclusive,accurate and reliable.And it can lay the foundation for follow-up study.