Pre-column Derivatives HPLC Fingerprint of Polygonati Rhizoma Polysaccharide Hydrolysate

Abstract:

Objective: To establish a pre-column derivation HPLC method of chromatographic fingerprint of Polygonati Rhizoma polysaccharide. Method: Polygonati Rhizoma polysaccharide was hydrolyzed with trifluoroacetic acid and derivated by 1-phenyl-3-methyl-5-pyrazolone (PMP). The monosaccharide composition was separated by reversed-phase technique on a Zorbax Eclipse XDB-C₁₈ column (4.6 mm×250 mm, 5 μm) with a mobile phase composed of 0.1 mol·L^-1phosphate buffer (pH 6.7) and acetonitrile in the ratio of 84.5 : 15.5.The flow rate was 0.8 mL·min^-1. The detection wavelength was set at 250 nm, and column temperature was at 30 ℃. Result: There were 10 common peaks from the fingerprint and 7 peaks were identified as D-mannose, L-rhamnose, D-galacturonic, D-galactose, D-glucose, D-xylose, and D-arabinose. The similarity of 10 batches of Polygonati Rhizoma polysaccharide was more than 0.99. Conclusion: The method is accurate and reproducible, which can be used for the quality control of Polygonati Rhizoma.