HPLC Determination of Gallic Acid and Quercetin in Archidendri Folium from Different Producing Areas

Abstract:

Objective: To establish a determination method for gallic acid and quercetin in Archidendri Folium from different producing areas. Method: HPLC was performed on Odyssil C₁₈ column (4.6 mm×250 mm, 5 μm), gradiently eluting with acetonitrile as mobile phase A and 0.2% H₃PO₄ as B at a flow rate of 1.0 mL·min^-1. The column temperature was 30 ℃. The detection wavelength was 254 nm. The injection volume was 5 μL. Result: The linearity of gallic acid was good in the range of 0.060 2-1.806 μg (r=0.999 5), and the average recovery was 97.27% (RSD 1.4%). The linearity of quercetin was good in the range of 0.016 9-0.507 6 μg (r=0.999 6), and the average recovery of 96.29%(RSD 1.3%). Conclusion: The established method is simple, accurate and can be used for the determination of gallic acid and quercetin in Archidendri Folium, it can able to provide reference for quality control of Archidendri Folium.