Determination of Total Flavonoids in Abrus mollis

Abstract:

Objective: To establish an exclusive method to determine the content of total flavonoids in Abrus mollis. Method: UV-VIS spectrophotometry was used to study the absorption spectrum changes of flavonoids in triethylamine (TEA) chromogenic agent with different concentrations of ethanol aqueous solution medium. Result: There was obvious difference between different flavonoids before and after coloration. The TEA method could be used to determine the content of apigenin flavonoids and and their absorption spectra were remarkably influenced by different concentrations of ethanol aqueous solution with the stable absorption wavelength of 400 nm after coloration in 50% ethanol aqueous solution. After the methodological study, 6-C-glycopyranosyl-8-C-glycopyranosyl apigenin had a good linearity in the ringe of 0.507-101.4 mg·L^-1(r=0.999 5), the average recovery was 98.01%with RSD of 2.1%. Conclusion: The content of total apigenin flavonoids in A. mollis can be determined by the TEA method in 50% EtOH aqueous solution with good specificity, accuracy and simple operation.