Transitional Features of Benzofuran Glycosides Part from Psoraleae Fructus in Rat Plasma

Abstract:

Objective: To invesitgate transitional features of rat plasma after administration of benzofuran glycosides part from Psoraleae Fructus. Method: A HPLC-UV method was established to determine contents of psoralenoside and isopsoralenoside in rat plasma after administration of benzofuran glycosides part,mobile phase composed of acetonitrile and 0.1% formic acid water in gradient elution,detection wavelength was set at 246 nm.Plasma concentration-time data were dealed with SPSS 11.0 software. Result: Psoralenoside and isopsoralenoside appeared in plasma after 15 min of administration of benzofuran glycosides with 0.003 g·g^-1,and reached the peak concentration in 1.5 h,also their metabolites (psoralen and isopsoralen) were found in plasma. Conclusion: Specificity and characteristic of plasma HPLC are good,which is successfully used for quantification of psoralenoside and isopsoralenoside in rat plasma after administration of benzofuran glycosides part,benzofuran glycosides can be transformed into corresponding coumarins in rat.