Effect of Ruanjian Sanjie Prescription on Pharmacokinetic Behavior of 5-Fluorouracil in Rats

Abstract:

Objective: To analysis effect of Ruanjian Sanjie (RJ) prescription on pharmacokinetic behavior of 5-fluorouracil (5-Fu) in rats. Method: Rats were divided into two groups,one group was given 5-Fu only and the other one was given 5-Fu and RJ prescription together.Plasma samples were separated and extracted by 10% perchloric acid solution,HPLC was employed to determine the content of 5-Fu with mobile phase of 0.01 mol·L^-1 potassium dihydrogen phosphate,a flow rate of 0.8 mL·min^-1 and detection wavelength at 265 nm.Pharmacokinetic parameters were calculated by WinNonlin 5.2.1 software with non-compartmental model. Result: RJ prescription could obviously reduce T_max [the combined group was (0.381±0.075) h and the single group was (0.514±0.122) h] and apparent volume of distribution [the combined group was (5.915±1.196) mL and the single group was (11.307±5.393) mL] of 5-Fu in rats,but no obvious changes on other parameters. Conclusion: RJ prescription can promote absorption of 5-Fu in rats by intraperitoneal injection,while inhibiting trend of 5-Fu distribution from blood to tissues.