Abstract:Objective: To observe the action of Bushen Jianpi decoction (BSJP) on the adipogenic differentiation potential of bone marrow mesenchymal stem cells(BMSCs) in tail suspension model rats. Method: Rats were assigned to five groups randomly:control(C),tail suspended(S),high dose of BSJP (H), middle dose of BSJP (M) and low dose of BSJP (L) groups. Rats in S, H, M and L groups were suspended by head down tilt -30° for 21 days to simulate weightlessness. Rats of H, M and L groups were orally administrated with BSJP and other groups with equivalent saline from the first day. BMSCs were isolated and cultured by adherence method, and the third generation cells were induced by adipogenic differentiation solution. The lipid droplet formation was detected by oil red O staining and the expression of peroxisome proliferators-activated receptor γ (PPARγ) was analyzed by Real time PCR and Western blot. The content of triglycerides(TG) in cells was examined on day 7, 14, 21 of adipogenic differentiation. Result: Compared with the C group, the content of TG was increased significantly on day 7, 14, 21 of adipogenic differentiation, the adipogenic differentiation potential and the mRNA and protein expression of PPARγ were also increased on day 21 of adipogenic differentiation in S group.Compared with the S group, the content of TG was declined on day 7, 14, 21 of adipogenic differentiation in BSJP H, M and L dose groups, the adipogenic differentiation potential and the mRNA and protein expression of PPARγ were also decreased on day 21 of adipogenic differentiation in the three groups. Compared with the M group, the content of TG on day 14, 21 of adipogenic differentiation were reduced in H group, the adipogenic differentiation potential and the expression of PPARγ were decreased in H group. Conclusion: The BSJP can inhibit the adipogenic differentiation of BMSCs by down-regulating the expression of PPARγ and the inhibition effect of high dose in the BSJP is best.
