Effect of Aconite containing Serum on L-type Calcium Channel in Rat Ventricular Myocytes

Abstract:

Objective: To investigate the effect of aconite containing serum on L-type calcium channel in isolated rat ventricular myocytes. Method: Aconite decoction 15.6 g·kg^-1 was were ig given rats 7 d to prepare containing serum;The concentration of containing drug serum was 10% to obtain rat ventricular myocyte, enzymatic dissociation was used,whole cell patch clamp technique was used to record the L-type calcium channel current, L-type calcium channel current amplitude and kinetics characteristics were observed. Result: In the blank serum group and drug serum group the average peak current density and average activation after administration were respectively (-3.59±1.06) pA/pF,12.87%(n=8, P <0.05)and (-4.01±0.88) pA/pF,51.17%(n=8, P <0.01). In aconite containing serum before and after the treatment V_1/2 and K of activation curve were respectively (-12.66±0.44) mV and (5.70±0.35);(-14.71±0.88) mV and (5.68±0.72)(P <0.05, n=8).In aconite containing serum before and after the treatment V_1/2 and K of inactivation curve were respectively (-28.10±0.20) mV,and (6.31±0.18) mV;(-37.55±0.11) mV and (5.94±0.09) (P <0.05, n=8).Aconite containing serum recovery curves before and after medication for the τ:(171.01±11.13)ms and(160.15±4.97)ms(n=8). Conclusion: Aconite containing serum has activate function to the myocardial cells of L-type calcium channel currents.