Effects Zuogui Wan on mRNA Expression of 11 β-HSD1 in Placenta of Gestational Diabetes Mellitus Rats

Abstract:

Objective: To study the effects of Zuogui Wan on the mRNA expression of 11 β-hydroxysteroid dehydrogenase type 1(11 β-HSD1) in placenta of gestational diabetes mellitus (GDM) rats. Method: Pregnant reproduction age SD rats were randomly divided into 5 groups:the normal pregnancy (C) group, the GDM control (GDM) group, the diabetes insulin treated (INS) group, Zuogui Wan treated (ZGW) group and Zuogui Wan combined insulin treated (F) group. The GDM was induced except the C group by injecting streptozocin (STZ) abdominally at 45 mg·kg^-1. The rats in the three treated groups received the treatment of Zuogui Wan and/or insulin from injecting STZ until 19-day pregnant. Glycemia was detected by using the glucose oxidase method. The corticosterone and insulin in blood serum were detected by using ELISA kit. The mRNA expression level of 11 β-HSD1 in placenta was evaluated by RT-qPCR, and the quantitative analysis was conducted. Result: Compared with the C group, the glycemia, serum insulin, corticosterone level and placental 11 β-HSD1 mRNA expression level increased in the GDM group (P <0.01). Compared with the GDM group, the glycemia level, serum insulin, corticosterone level and placental 11 β-HSD1 mRNA expression level decreased significantly in AGW and F groups, but the results were higher than those in the C group. Moreover, the effect is better in F group. Conclusion: Zuogui Wan could reduce the level of glycemia in diabetic rats. The mRNA expression of 11 β-HSD1 was found in placental and could be influenced by GDM. Zuogui Wans have the improving effects on the glycemic status of GDM rats, which may be related to reducing the placental 11 β-HSD1 gene expression level.