Abstract:Objective: To study the effect of ShanJing capsule on nuclear transcription factor-κB (NF-κB) signal pathway relating apoptosis proteins caspases-3 (Caspase-3), anti-apoptotic genes B cell lymphoma/leukemia-2(Bcl-2), pro-apoptotic genes Bcl-2 associated X protein(Bax), explore its protective effects on myocardial ischemia and mechanisms. Method: Wistar rats the coronary artery of which were ligated were used for myocardial ischemia model, the changes of the ST section in electrocardiogram(ECG) were observed, and randomly divided into 5 groups:model (0.9% sodium chloride), Shanjing capsule high, medium and low dose (respectively 283.5, 850.5,1 701.0 mg·kg-1), Metoprolol capsules(324.0 mg·kg-1), compared with sham group (0.9% sodium chloride). All groups were administered once daily for 10 days. The ST segment,serum lactate dehydrogenase (LDH), creatine kinase (CK), aspartate aminotransferase (AST) levels were observed. Immunohistochemistry was used to detect the NF-κB, Caspase-3, Bax, Bcl-2 protein of myocardial tissue. Result: Compared with the sham group, ST segment elevation was significantly raised (P <0.05), LDH, CK and AST activity was significantly higher (P <0.05), NF-κB, Caspase-3, Bax's protein expression levels of myocardial tissue were significantly higher (P <0.05), Bcl-2 protein expression was significantly lower (P <0.05) in the model group;Shanjing capsule could obviously ameliorate the raise of ST segment, and lower activity of CK, LDH and AST. Shanjing capsule could obviously decrease the protein expressions of NF-κB, Caspase-3 and Bax and increase the protein expression of Bcl-2. Conclusion: Shanjing capsule could regulate the expressions of NF-κB signaling pathway relating proteins, inhibit the myocardial apoptosis and reduce ischemia anoxic pathological damage of myocardial muscle cells to protect the myocardial muscle.
