Toxicity-reducing Effect of Compatibility of Aconiti Lateralis Radix Praeparata with Different Proportion of Panax Ginseng in Neonatal Rat Cardiomyocytes

Abstract:

Objective: In order to evaluate the inducing toxicity effect of Aconiti Lateralis Radix Praeparata and different compatibility proportion of Ginseng Radix et Rhizoma in neonatal rats cardiomyocytes. Method: The primary culture cardiac cells (6 d) were randomly divided into normal(NC) group, Aconiti Lateralis Radix Praeparata and Ginsang Radix et Rhizoma(AG) group, blood serum(BS) group. The inducing toxicity effect of blood serum of different compatibility proportion of Aconiti Lateralis Radix Praeparata and Ginseng Radix et Rhizoma on spontaneous imp frequency, cell viability, superoxde dismutase(SOD), malondialdehyde(MDA), Nitric oxide (NO) and lactate dehydrogenase(LDH) was investigated in vitro in neonatal rat cardiomyocytes. The activity of Caspases-3, the expression of Bcl-2 and Bax protein, the expression of Bcl-2 and Caspases-3 mRNA was assayed by spectrophotometry, western blot and real-time PCR respectively. Result: Compared with AG_1:0 group, the content of SOD increased obviously, spontaneous imp frequency, the content of MDA, the secretory volume of NO and the release rate of LDH decreased obviously, the activity of Caspases-3, the expression of Bax protein and Caspases-3 mRNA decreased obviously, the expression of Bcl-2 protein and Bcl-2 mRNA increased gradually, cell viability increased in 2-4 h significantly (P <0.05) in other AG groups. Compared with BS group, there was no significant difference in AG_{1 : 0.5} group in the above indexes. Conclusion: There was a significant inducing toxicity effect of Aconiti Lateralis Radix Praeparata and different compatibility proportion of Ginseng Radix et Rhizoma in neonatal rats cardiomyocytes, the optimization compatibility proportion in AG_1:0.5.