Study on the HPLC fingerprints of Glochidion eriocarpumChamp in Guangxi

Abstract:

Abstract: OBJECTIVE To establish the chromatographic fingerprint of Glochidion eriocarpumChamp by HPLC. METHODS The fingerprint was investigated using HypersilC18 (250mm×4.6mm,5μm). The mobile phase consisted of Methanol and 0.2% Phosphoric acid, gradient elution 0~7min (10%B), 7~15min (10%B→22%B), 15~20min(22%B→30%B), 20~30min(30%B→40%B), 30~45min(40%B). The detection wavelength was 270 nm, column temperature was 30℃, The flow rate was 1mL?min-1. The fingerprints of 10 batches of Glochidion eriocarpumChamp were analyzed in 45 min. The similarity was analyzed by similarity evaluation system for chromatographic fingerprint of TCM (Version 2004 A). RESULTS The HPLC fingerprint of Guangxi Glochidion eriocarpumChamp was obtained. The similarity was good. CONCLUSION The method was simple, reproducible and can be used in quality control for Glochidion eriocarpumChamp.