Study on HPLC Fingerprint of Tibetan Medicine Pterocephali Herba

Abstract:

Objective: To establish HPLC fingerprint of Tibetan medicine Pterocephali Herba, and compare it of Pterocephali Herba from different place, provided a new methods for its quality evaluation. Method: Adoption the method of HPLC to determine 10 groups of Pterocephali Herba from different place. Chromatographic Column was Boston Symmetrix(250×4.6mm,5μm), mobile phase was Methanol-0.1 mol.L_-1 ammonium acetate solution(81:19),velocity was 0.8mL.min_-1, the detection wavelength was 210nm, column temperature was 30℃. And compared the similarity of Pterocephali Herba from different place. Result: Taking advantage of < USP fingerprint similarity evaluation system (2004A version)> to establish common mode of HPLC fingerprint of Tibetan medicine Pterocephali Herba, and marked 101 characteristic peaks. The 10 groups samples had high similarity. Conclusion: This method had good repeatability, precision, and stability, providing scientific basis for quality control of Pterocephali Herba.