Chinese Journal of Experimental Traditional Medical Formulae
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2013, 19(20):246-250.
DOI: 10.11653/syfj2013200246
Abstract:
Objective: To investigate the intervention effect and potential mechanism of Qingre Huashi decoction(QHD) for preventing the onset of colon cancer by observing SGC-7901 cell proliferation and apoptosis along with the expression of CD14 gene, tumor necrosis factor alpha and interleukin 1 beta. Method: SGC-7901 cells were divided into control group, intervention group. In vitro, the control group was offered fetal calf serum. The intervention group was fed with serum containing QHD for 5%,10%,15%,20%,30%. The multiplication, proliferation and apoptosis of SGC-7901 cell were detected by MTT and flow cytometry respectively. The expression of CD14, TNF-α and IL-1β gene was assayed by immunohistochemistry and real-time PCR. Result: Compared with control group, the survival of SGC-7901 cell was decreased in QHD group(P<0.05). In QHD group, the cells in G0/G1 phase were increased but the S phase cell was on the decline. Those changes were correlated with the culturing content. There is a significant difference between QHD group and control group in those changes as mentioned above(P<0.05). Especially, the effect of middle concentration serum containing QHD on inducing cell apoptosis and down regulating the expression of CD14, TNF-α, IL-1β was obvious (P<0.05). Conclusion: QHD exerts a distinct effect on preventing the onset of colon cancer by mediating SGC-7901 cell S time, cell composition and regulating the expression of CD14, TNF-α and IL-1β and inducing cell apoptosis.
Objective: To investigate the intervention effect and potential mechanism of Qingre Huashi decoction(QHD) for preventing the onset of colon cancer by observing SGC-7901 cell proliferation and apoptosis along with the expression of CD14 gene, tumor necrosis factor alpha and interleukin 1 beta. Method: SGC-7901 cells were divided into control group, intervention group. In vitro, the control group was offered fetal calf serum. The intervention group was fed with serum containing QHD for 5%,10%,15%,20%,30%. The multiplication, proliferation and apoptosis of SGC-7901 cell were detected by MTT and flow cytometry respectively. The expression of CD14, TNF-α and IL-1β gene was assayed by immunohistochemistry and real-time PCR. Result: Compared with control group, the survival of SGC-7901 cell was decreased in QHD group(P<0.05). In QHD group, the cells in G0/G1 phase were increased but the S phase cell was on the decline. Those changes were correlated with the culturing content. There is a significant difference between QHD group and control group in those changes as mentioned above(P<0.05). Especially, the effect of middle concentration serum containing QHD on inducing cell apoptosis and down regulating the expression of CD14, TNF-α, IL-1β was obvious (P<0.05). Conclusion: QHD exerts a distinct effect on preventing the onset of colon cancer by mediating SGC-7901 cell S time, cell composition and regulating the expression of CD14, TNF-α and IL-1β and inducing cell apoptosis.
2015, 21(11):7-10.
DOI: 10.13422/j.cnki.syfjx.2015110007
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Objective: To investigate correlation between content and anti-proliferative effect against lung carcinoma cell line SPC-A-1 and A549 of active ingredients in Coicis Semen from five places. Method: HPLC-ELSD and UV spectrophotometry were employed to determine contents of glycerol trioleate and total triglycerides in Coicis Semen,respectively.In addition,MTT assay was performed to explore anti-proliferative effect of Coicis Semen extract from various origins towards SPC-A-1 and A549 cells. Result: The content of glycerol trioleate in Coicis Semen from Hebei,Liaoning,Fujian,Guizhou,Shandong province was 0.91%,1.14%,0.82%,0.77%,1.09%,the content of total triglycerides was 14.83,10.88,7.85,5.26,7.71 mg·g-1,respectively.IC50(median inhibitory concentration) against the lung carcinoma cell line A549 were 2.33,2.77,3.15,3.24,3.08 g·L-1,IC50 against SPC-A-1 were 1.64,2.15,2.63,3.01,2.99 g·L-1,respectively. Conclusion: All samples originated from five provinces are in line with standards of the 2010 edition of Chinese Pharmacopoeia.However,samples from Hebei province have the highest content of total triglycerides and the strongest anti-lung cancer activity,suggesting a positive correlation between active ingredients content and pharmacological activity.
Objective: To investigate correlation between content and anti-proliferative effect against lung carcinoma cell line SPC-A-1 and A549 of active ingredients in Coicis Semen from five places. Method: HPLC-ELSD and UV spectrophotometry were employed to determine contents of glycerol trioleate and total triglycerides in Coicis Semen,respectively.In addition,MTT assay was performed to explore anti-proliferative effect of Coicis Semen extract from various origins towards SPC-A-1 and A549 cells. Result: The content of glycerol trioleate in Coicis Semen from Hebei,Liaoning,Fujian,Guizhou,Shandong province was 0.91%,1.14%,0.82%,0.77%,1.09%,the content of total triglycerides was 14.83,10.88,7.85,5.26,7.71 mg·g-1,respectively.IC50(median inhibitory concentration) against the lung carcinoma cell line A549 were 2.33,2.77,3.15,3.24,3.08 g·L-1,IC50 against SPC-A-1 were 1.64,2.15,2.63,3.01,2.99 g·L-1,respectively. Conclusion: All samples originated from five provinces are in line with standards of the 2010 edition of Chinese Pharmacopoeia.However,samples from Hebei province have the highest content of total triglycerides and the strongest anti-lung cancer activity,suggesting a positive correlation between active ingredients content and pharmacological activity.
2014, 20(5):54-56.
DOI: 10.11653/syfj2014050054
Abstract:
Objective: To establish a high performance liquid chromatographic method (HPLC) for simultaneous determination of rubrofusarin gentiobioside, casside, cassiaside C in Cassia obtusifolia. Method: The HPLC with Dionex Acclaim C18(4.6 mm×250 mm, 5 μm) column was used, acetonitrile-THF-1% acetic acid (17 ：2 ：81) was used as a mobile phase, with flow rate of 1 mL·min-1, column temperature at 30 ℃ and detection wavelength at 278 nm. Result: Rubrofusarin gentiobioside, casside, cassiaside C showed good linearity (r=0.999 9) in the range of 0.218-1.09, 0.188-0.94, 0.200-1.000 μg, regressive equation were Y=54.017X-0.344 6 (r=0.999 9),Y=78.63X-0.354(r=0.999 9),Y=74.098X-0.433 5 (r=0.999 9)respectively;the average recoveries of the method were 98.43%, 101.89%, 102.46%, RSD were 1.75%, 1.10%, 1.15%. Conclusion: The method was proved to be simple, rapid, sensitive, precise, reliable and repeatable. It can be applied to the quality control of Semen Cassia.
Objective: To establish a high performance liquid chromatographic method (HPLC) for simultaneous determination of rubrofusarin gentiobioside, casside, cassiaside C in Cassia obtusifolia. Method: The HPLC with Dionex Acclaim C18(4.6 mm×250 mm, 5 μm) column was used, acetonitrile-THF-1% acetic acid (17 ：2 ：81) was used as a mobile phase, with flow rate of 1 mL·min-1, column temperature at 30 ℃ and detection wavelength at 278 nm. Result: Rubrofusarin gentiobioside, casside, cassiaside C showed good linearity (r=0.999 9) in the range of 0.218-1.09, 0.188-0.94, 0.200-1.000 μg, regressive equation were Y=54.017X-0.344 6 (r=0.999 9),Y=78.63X-0.354(r=0.999 9),Y=74.098X-0.433 5 (r=0.999 9)respectively;the average recoveries of the method were 98.43%, 101.89%, 102.46%, RSD were 1.75%, 1.10%, 1.15%. Conclusion: The method was proved to be simple, rapid, sensitive, precise, reliable and repeatable. It can be applied to the quality control of Semen Cassia.
2014, 20(9):15-18.
DOI: 10.13422/j.cnki.syfix.2014090015
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Objective: To optimize extraction technology of Jiedu Tongluo particles. Method: Based on single factor tests, central composite design-response surface methodology was applied to optimize extraction process with soaking time, decoction time and solvent amounts as independent variables, while composite score of extraction amounts of baicalin, quercetin and isorhamnetin as dependent variable.SPSS software was used to fit multivariate linear equation and second-order polynomial equation for experimental data, response surface and contour plot were delineated according to best-fit mathematic models by Design Expert 8.0.0 statistic analysis software. Result: Optimum extraction process was as follows:soaking time 90 min, decoction time 85.63 min, the amount of solvent 9.21 times;Composite score was (93.2±0.051) %, which had deviation of -2.32% with the predicted value of 94.30%. Conclusion: Model established by central composite design-response surface methodology had good predictability, which was suitable for optimizing extraction process of Jiedu Tongluo particles.
Objective: To optimize extraction technology of Jiedu Tongluo particles. Method: Based on single factor tests, central composite design-response surface methodology was applied to optimize extraction process with soaking time, decoction time and solvent amounts as independent variables, while composite score of extraction amounts of baicalin, quercetin and isorhamnetin as dependent variable.SPSS software was used to fit multivariate linear equation and second-order polynomial equation for experimental data, response surface and contour plot were delineated according to best-fit mathematic models by Design Expert 8.0.0 statistic analysis software. Result: Optimum extraction process was as follows:soaking time 90 min, decoction time 85.63 min, the amount of solvent 9.21 times;Composite score was (93.2±0.051) %, which had deviation of -2.32% with the predicted value of 94.30%. Conclusion: Model established by central composite design-response surface methodology had good predictability, which was suitable for optimizing extraction process of Jiedu Tongluo particles.
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【Abstract】Objective To study the mechanism of the chinese medicine Weichangshu on mitochondrial kinetic energy in diabetic mellitus rats stomach by measuring the changes of morphology, structure, function and protein of mitochondria. Methods: Standard Sprague Dawley rats with a mean weight of 150-170g were randomly divided into 4 groups, including a control group, DM model group, DM WeichangshuⅠgroup, DM Weichangshu Ⅱ group. The mitochondrial morphology and structure were detected using transmission electron microscopy. The changes of mitochondrial transmembrane potential, respiratory chain and mitochondrial protein were detected using flow cytometry, fluorescence labeling and Western Blot respectively. Results: Weichangshu could ameliorate the mitochondrial morphology, structure and respiratory chain in diabetic mellitus rats stomach. Mitochondrial transmembrane potential, ATP level and activity of cytochrome C oxidase were increased. The release of ROS was decreased. The expression of Bcl-2 up-regulated and bax down-regulated. Conclusion: Weichangshu could promoted the gastrointestinal motility, which might be related with the regulation of mitochondrial kinetic energy.
【Abstract】Objective To study the mechanism of the chinese medicine Weichangshu on mitochondrial kinetic energy in diabetic mellitus rats stomach by measuring the changes of morphology, structure, function and protein of mitochondria. Methods: Standard Sprague Dawley rats with a mean weight of 150-170g were randomly divided into 4 groups, including a control group, DM model group, DM WeichangshuⅠgroup, DM Weichangshu Ⅱ group. The mitochondrial morphology and structure were detected using transmission electron microscopy. The changes of mitochondrial transmembrane potential, respiratory chain and mitochondrial protein were detected using flow cytometry, fluorescence labeling and Western Blot respectively. Results: Weichangshu could ameliorate the mitochondrial morphology, structure and respiratory chain in diabetic mellitus rats stomach. Mitochondrial transmembrane potential, ATP level and activity of cytochrome C oxidase were increased. The release of ROS was decreased. The expression of Bcl-2 up-regulated and bax down-regulated. Conclusion: Weichangshu could promoted the gastrointestinal motility, which might be related with the regulation of mitochondrial kinetic energy.
2012, 18(24):39-41.
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Objective:To optimize matrix prescription of Guyanxiao cataplasm. Method: Matrix prescription was optimized by orthogonal test with initial viscosity,sustained adhesive strength and general sensory as comprehensive evaluation index,the amount of NP700,CMC-Na, glycerol and titania were chosen as factors. Result: Optimal matrix prescription was:NP-700, CMC-Na, glycerol and titania of 3,0.6,16,0.8g,respectively. Conclusion: Guyanxiao catapasm with good appearance and adhesion could be prepared by optimized matrix formulation.
Objective:To optimize matrix prescription of Guyanxiao cataplasm. Method: Matrix prescription was optimized by orthogonal test with initial viscosity,sustained adhesive strength and general sensory as comprehensive evaluation index,the amount of NP700,CMC-Na, glycerol and titania were chosen as factors. Result: Optimal matrix prescription was:NP-700, CMC-Na, glycerol and titania of 3,0.6,16,0.8g,respectively. Conclusion: Guyanxiao catapasm with good appearance and adhesion could be prepared by optimized matrix formulation.
2013, 19(8):46-50.
DOI: 10.11653/syfj2013080046
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Objective: To optimize formulation of ursolic acid self-microemulsion drug delivery system using central composite design/response surface methodology. Method: With ratio of surfactants and cosurfactants and oil content as independent variables,particle size and saturated drug doading were taken as dependent variables,optimum formulation was screened by SAS9.1.3 software,and verified. Result: Optimized formulation of ursolic acid self-microemulsion was oil phase(MCT)-emulsifier(HS15)-cosurfactants(alcohol) 12.5:62.5:25. Conclusion: This optimized formulation of ursolic acid self-microemulsion drug delivery system could be obtained exactly and conveniently by central composite design response surface methodology,and established model had a reliable predictability.
Objective: To optimize formulation of ursolic acid self-microemulsion drug delivery system using central composite design/response surface methodology. Method: With ratio of surfactants and cosurfactants and oil content as independent variables,particle size and saturated drug doading were taken as dependent variables,optimum formulation was screened by SAS9.1.3 software,and verified. Result: Optimized formulation of ursolic acid self-microemulsion was oil phase(MCT)-emulsifier(HS15)-cosurfactants(alcohol) 12.5:62.5:25. Conclusion: This optimized formulation of ursolic acid self-microemulsion drug delivery system could be obtained exactly and conveniently by central composite design response surface methodology,and established model had a reliable predictability.
2014, 20(8):15-18.
DOI: 10.13422/j.cnki.syfix.2014080015
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Objective: To optimize ultrafine grinding process of Chuanxiong Rhizoma. Method: Taking feeding amount, moisture content and grinding time as independent variables, average particle size as dependent variable, Box-Behnken response surface methodology was adopted to optimizeultrafine grinding process of Chuanxiong Rhizoma, its powder properties and hygroscopicity before and after smashed were investigated. Result: Optimum ultrafine grinding process was as follows:feeding amount1.3 kg, moisture content 4%, grinding time 19 min;Deviation between the predicted value(18.357 7 μm) and the measured value(19.187 μm) of average particle size was only 4.5%.Response angles of ordinary and ultrafine powder were (49.4±0.03) and (50.2±0.02) °, bulk densities were (0.429±0.003) and (0.363±0.005) g·mL-1, tap densities were (0.609±0.05) amd (0.556±0.10) g·mL-1, moisture equilibrium times were about 80 h. Conclusion: Optimized ultrafine grinding process was reasonable and feasible, preparation technology of ultrafine powder of Chuanxiong Rhizoma should add flow aid and other auxiliary materials in order to improve fluidity of powder, ultrafine grinding process should strictly control humidity.
Objective: To optimize ultrafine grinding process of Chuanxiong Rhizoma. Method: Taking feeding amount, moisture content and grinding time as independent variables, average particle size as dependent variable, Box-Behnken response surface methodology was adopted to optimizeultrafine grinding process of Chuanxiong Rhizoma, its powder properties and hygroscopicity before and after smashed were investigated. Result: Optimum ultrafine grinding process was as follows:feeding amount1.3 kg, moisture content 4%, grinding time 19 min;Deviation between the predicted value(18.357 7 μm) and the measured value(19.187 μm) of average particle size was only 4.5%.Response angles of ordinary and ultrafine powder were (49.4±0.03) and (50.2±0.02) °, bulk densities were (0.429±0.003) and (0.363±0.005) g·mL-1, tap densities were (0.609±0.05) amd (0.556±0.10) g·mL-1, moisture equilibrium times were about 80 h. Conclusion: Optimized ultrafine grinding process was reasonable and feasible, preparation technology of ultrafine powder of Chuanxiong Rhizoma should add flow aid and other auxiliary materials in order to improve fluidity of powder, ultrafine grinding process should strictly control humidity.
2012, 18(24):24-27.
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Objective:To optimize matrix formulation of Sanhuang Sanyu cataplasm. Method: Taking appearance and adhesion of cataplasm as evaluation indexes,matrix formulation of Sanhuang Sanyu cataplasm was optimized by single factor test and orthogonal test. Result: Optimized matrix formulation of Sanhuang Sanyu cataplasm was NP-700-K90-glycerol(0.5∶0.4∶6).0.02 g·mL-1 solution was received by adding carbomer-u10NF into mixed extraction concentrated liquid of Rheum palmatum and Scutellaria baicalensis,swelling overnight, as A phase,0.5 g sodium polyacrylate and 0.4 g PVP were added to 6 g glycerol as B phase,aluminum chloride and citric acid were added to water as C phase,single extraction concentrated liquid of Phellodendron chinense was as D phase,four phases was fully mixed with A-B-C-D(10∶3∶3∶5), stirred 15 min, coating,covered with anti-sticking layer. Conclusion: Optimized matrix formulation of Sanhuang Sanyu cataplasm was simple,stable and feasible,which could be applied to industrial production.
Objective:To optimize matrix formulation of Sanhuang Sanyu cataplasm. Method: Taking appearance and adhesion of cataplasm as evaluation indexes,matrix formulation of Sanhuang Sanyu cataplasm was optimized by single factor test and orthogonal test. Result: Optimized matrix formulation of Sanhuang Sanyu cataplasm was NP-700-K90-glycerol(0.5∶0.4∶6).0.02 g·mL-1 solution was received by adding carbomer-u10NF into mixed extraction concentrated liquid of Rheum palmatum and Scutellaria baicalensis,swelling overnight, as A phase,0.5 g sodium polyacrylate and 0.4 g PVP were added to 6 g glycerol as B phase,aluminum chloride and citric acid were added to water as C phase,single extraction concentrated liquid of Phellodendron chinense was as D phase,four phases was fully mixed with A-B-C-D(10∶3∶3∶5), stirred 15 min, coating,covered with anti-sticking layer. Conclusion: Optimized matrix formulation of Sanhuang Sanyu cataplasm was simple,stable and feasible,which could be applied to industrial production.
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Fracture healing is a process that involves the proliferation and differentiation of cells and tissues and can be stimulated by drugs. Traditional medicine has a reputation in the therapy on bone fracture. It is believed that herbal formulae promotes bone repair through multiple functions of “anti-inflammation”, “promoting blood circulation” and “stimulating bone formation”. This study aims to engineer a herbal formula under the guidance of Chinese medicine theories, then define the effective dosage ratio between the herbs and demonstrate the biological effects of the formulae on different parameters of healing using relevant in vitro assays. Six herbs which are frequently used for fractures were combined into five formulae according to the “Uniform Design” method. Assays of nitric oxide (NO) inhibition in mouse macrophages, human umbilical vein endothelial cells (HUVEC) and rat osteoblasts proliferation were used to reflect the “anti-inflammation inflammatory”, “circulation promotion” and “bone formation stimulation” functions. The results showed that all the formulae expressed significant activities of NO inhibition, and HUVEC cell and osteoblasts proliferation. Formula 5 with the largest doses (containing 30g Radix et Rhizoma Rhei, 9g Fructus Gardeniae, 9g Radix et Rhizoma Notoginseng, 9g Flos Carthami, 30g Ramulus Sambucus Willamsii, 15g Radix Dipsaci) demonstrated the best results in all the three assays.
Fracture healing is a process that involves the proliferation and differentiation of cells and tissues and can be stimulated by drugs. Traditional medicine has a reputation in the therapy on bone fracture. It is believed that herbal formulae promotes bone repair through multiple functions of “anti-inflammation”, “promoting blood circulation” and “stimulating bone formation”. This study aims to engineer a herbal formula under the guidance of Chinese medicine theories, then define the effective dosage ratio between the herbs and demonstrate the biological effects of the formulae on different parameters of healing using relevant in vitro assays. Six herbs which are frequently used for fractures were combined into five formulae according to the “Uniform Design” method. Assays of nitric oxide (NO) inhibition in mouse macrophages, human umbilical vein endothelial cells (HUVEC) and rat osteoblasts proliferation were used to reflect the “anti-inflammation inflammatory”, “circulation promotion” and “bone formation stimulation” functions. The results showed that all the formulae expressed significant activities of NO inhibition, and HUVEC cell and osteoblasts proliferation. Formula 5 with the largest doses (containing 30g Radix et Rhizoma Rhei, 9g Fructus Gardeniae, 9g Radix et Rhizoma Notoginseng, 9g Flos Carthami, 30g Ramulus Sambucus Willamsii, 15g Radix Dipsaci) demonstrated the best results in all the three assays.
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Abstract:Objective: to investigate the absorption mechanism of palmatine chloride by using Caco-2 monolayer mode1.Methods: Caco-2 cell monolayer model was used to investigate the bi-directional transport of palmatine chloride. The effect of time, drug concentration, temperature, pH and inhibitors on the absorption of palmatine chloride was studied. Drug concentration was measured by HPLC and the apparent permeability coefficients (Papp) were calculated. Results: The apical to basolateral permeability (Papp) of palmatine chloride was bigger than the basolateral to apical permeability(Papp).Transport of palmatine chloride was time and concentration dependent, and it was also effected by P-glycoprotein inhibitor, pH and temperature. Conclusion:The absorption of palmatine chloride in Caco-2 cell model was an active transportation mediated by transporter, which mainly located in the apical side of Caco-2 cell monolayer.
Abstract:Objective: to investigate the absorption mechanism of palmatine chloride by using Caco-2 monolayer mode1.Methods: Caco-2 cell monolayer model was used to investigate the bi-directional transport of palmatine chloride. The effect of time, drug concentration, temperature, pH and inhibitors on the absorption of palmatine chloride was studied. Drug concentration was measured by HPLC and the apparent permeability coefficients (Papp) were calculated. Results: The apical to basolateral permeability (Papp) of palmatine chloride was bigger than the basolateral to apical permeability(Papp).Transport of palmatine chloride was time and concentration dependent, and it was also effected by P-glycoprotein inhibitor, pH and temperature. Conclusion:The absorption of palmatine chloride in Caco-2 cell model was an active transportation mediated by transporter, which mainly located in the apical side of Caco-2 cell monolayer.
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Abstract：Objective To observe the effect of Dendrobium with different doses on ALT, AST, TC in the serum of chronic alcoholic liver injury mice. Methods Male ICR mice were divided into nine groups randomly: normal control group, model control group, positive control group, fresh Dendrobium groups with three doses(3g/kg,6g/kg,9g/kg), dried Dendrobium groups with three doses(0.45g/kg,0.90g/kg,1.35g/kg), gastric infusion for 65 days. The mice mode1 of chronic alcoholic 1iver injury was established by filling the stomach with alcohol after the administration for 65 days, once a day. The blood was taken to separate into serum and the activity of ALT, AST and the content of TC in the serum were determined. Results Compared with the normal control group, the ALT, AST, TC in the serum of model control group increased. Compared with model control group, fresh Dendrobium (3g/kg) and dried Dendrobium (0.45g/kg) could decrease ALT in the serum；fresh Dendrobium (3g/kg, 6g/kg) and dried Dendrobium (0.45g/kg) could decrease AST, TC in the serum; dried Dendrobium (0.90g/kg, 1.35g/kg) could decrease AST in the serum. Conclusion Fresh and dried Dendrobium could improve effectively the activity of ALT, AST and decrease the content of TC in the serum of chronic alcoholic 1iver injury mice.
Abstract：Objective To observe the effect of Dendrobium with different doses on ALT, AST, TC in the serum of chronic alcoholic liver injury mice. Methods Male ICR mice were divided into nine groups randomly: normal control group, model control group, positive control group, fresh Dendrobium groups with three doses(3g/kg,6g/kg,9g/kg), dried Dendrobium groups with three doses(0.45g/kg,0.90g/kg,1.35g/kg), gastric infusion for 65 days. The mice mode1 of chronic alcoholic 1iver injury was established by filling the stomach with alcohol after the administration for 65 days, once a day. The blood was taken to separate into serum and the activity of ALT, AST and the content of TC in the serum were determined. Results Compared with the normal control group, the ALT, AST, TC in the serum of model control group increased. Compared with model control group, fresh Dendrobium (3g/kg) and dried Dendrobium (0.45g/kg) could decrease ALT in the serum；fresh Dendrobium (3g/kg, 6g/kg) and dried Dendrobium (0.45g/kg) could decrease AST, TC in the serum; dried Dendrobium (0.90g/kg, 1.35g/kg) could decrease AST in the serum. Conclusion Fresh and dried Dendrobium could improve effectively the activity of ALT, AST and decrease the content of TC in the serum of chronic alcoholic 1iver injury mice.
2014, 20(23).
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Objective To establish a sensitive and specific HPLC method for quality control of Ilex hainanensis Merr.．Methods HPLC method was applied for quality assessment of Ilex hainanensis Merr.． HPLC analysis was performed on a HanbonC18 column (250mm×4．6mm，5 m)．The mobile phase consisted of acetonitrile (solvent B) and watercontaining0.1% (v/v) phosphoric acid (solvent A) at a constant flow rate of 1.0ml/min．An increasing linear gradient (v/v) of solvent B was used. The injection volume was 10μl. The column temperature was set at 25℃. The chromatogram s were monitored at 330nm. Results In different varieties of processing methods of Ilex hainanensis Merr. showing 9 characteristic peaks. It was established from its prepared samples of Ilex hainanensis Merr.．Conclusion The characteristic chromatogram of Ilex hainanensis Merr. with high specificity can be used to control its quality as well as the consistency of processing products.
Objective To establish a sensitive and specific HPLC method for quality control of Ilex hainanensis Merr.．Methods HPLC method was applied for quality assessment of Ilex hainanensis Merr.． HPLC analysis was performed on a HanbonC18 column (250mm×4．6mm，5 m)．The mobile phase consisted of acetonitrile (solvent B) and watercontaining0.1% (v/v) phosphoric acid (solvent A) at a constant flow rate of 1.0ml/min．An increasing linear gradient (v/v) of solvent B was used. The injection volume was 10μl. The column temperature was set at 25℃. The chromatogram s were monitored at 330nm. Results In different varieties of processing methods of Ilex hainanensis Merr. showing 9 characteristic peaks. It was established from its prepared samples of Ilex hainanensis Merr.．Conclusion The characteristic chromatogram of Ilex hainanensis Merr. with high specificity can be used to control its quality as well as the consistency of processing products.
2014, 20(23).
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Objective: To establish HPLC fingerprint of Tibetan medicine Pterocephali Herba, and compare it of Pterocephali Herba from different place, provided a new methods for its quality evaluation. Method: Adoption the method of HPLC to determine 10 groups of Pterocephali Herba from different place. Chromatographic Column was Boston Symmetrix(250×4.6mm,5μm), mobile phase was Methanol-0.1 mol.L-1 ammonium acetate solution(81:19),velocity was 0.8mL.min-1, the detection wavelength was 210nm, column temperature was 30℃. And compared the similarity of Pterocephali Herba from different place. Result: Taking advantage of < USP fingerprint similarity evaluation system (2004A version)> to establish common mode of HPLC fingerprint of Tibetan medicine Pterocephali Herba, and marked 101 characteristic peaks. The 10 groups samples had high similarity. Conclusion: This method had good repeatability, precision, and stability, providing scientific basis for quality control of Pterocephali Herba.
Objective: To establish HPLC fingerprint of Tibetan medicine Pterocephali Herba, and compare it of Pterocephali Herba from different place, provided a new methods for its quality evaluation. Method: Adoption the method of HPLC to determine 10 groups of Pterocephali Herba from different place. Chromatographic Column was Boston Symmetrix(250×4.6mm,5μm), mobile phase was Methanol-0.1 mol.L-1 ammonium acetate solution(81:19),velocity was 0.8mL.min-1, the detection wavelength was 210nm, column temperature was 30℃. And compared the similarity of Pterocephali Herba from different place. Result: Taking advantage of < USP fingerprint similarity evaluation system (2004A version)> to establish common mode of HPLC fingerprint of Tibetan medicine Pterocephali Herba, and marked 101 characteristic peaks. The 10 groups samples had high similarity. Conclusion: This method had good repeatability, precision, and stability, providing scientific basis for quality control of Pterocephali Herba.
2014, 20(23).
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Objective: To establish TLC fingerprint of antibacterial extraction of Pilea aquarum Dunn. Methods: The antibacterial activity extraction of Pilea aquarum Dunn was confirmed by antibacterial activity research, and then the TLC fingerprint of 10 batches of Pilea aquarum Dunn from Guizhou was analyzed comparatively. Results: The ethyl acetate extraction of Pilea aquarum Dunn is antibacterial activity. Another, the TLC chromatogram of 10 groups of drugs were very similar, which had 8 spots and 8 corresponding peaks, but the intensity of each peak was different. Conclusion: The method is simple and stabilized, and can be used for the quality control of Pilea aquarum Dunn.
Objective: To establish TLC fingerprint of antibacterial extraction of Pilea aquarum Dunn. Methods: The antibacterial activity extraction of Pilea aquarum Dunn was confirmed by antibacterial activity research, and then the TLC fingerprint of 10 batches of Pilea aquarum Dunn from Guizhou was analyzed comparatively. Results: The ethyl acetate extraction of Pilea aquarum Dunn is antibacterial activity. Another, the TLC chromatogram of 10 groups of drugs were very similar, which had 8 spots and 8 corresponding peaks, but the intensity of each peak was different. Conclusion: The method is simple and stabilized, and can be used for the quality control of Pilea aquarum Dunn.
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Abstract:
Objecive To discuss the relationship between water-soluble sugar and cold-heat properties and furtherly reveal correlations of four properties and material basis.Methods Ten cold herbs and heat herbs were selected for the determination,and dealed with the water-soluble sugar with hydroxylamine hydrochloride,pyridine and acetic anhydride.Measuring Gas chomatography-mass(GC-MS) fingerprints of water-soluble sugar,then determining the water-soluble components of 20 herbs by mass spectrometry and analyzing the results by Fisher method.Results A discriminant function had been established by Fisher.The Fisher discriminant analysis could distinguish the cold and heat properties about 20 herbs that the group back substitution is 100%.From the results,it confirmes that there is difference between the cold and heat properties in the GC-MS fingerprints by Fisher method,which shows that there are different water-soluble sugar in the cold and the heat herbs.Conclusion The Fisher analysis could differentiate the cold and heat properties of herbs,and it is an effective statistical tool to study the relationship between the cold and heat properties and water-soluble sugar.It concludes that the water-soluble sugar is one of the material basis of four properties of herbs.
Objecive To discuss the relationship between water-soluble sugar and cold-heat properties and furtherly reveal correlations of four properties and material basis.Methods Ten cold herbs and heat herbs were selected for the determination,and dealed with the water-soluble sugar with hydroxylamine hydrochloride,pyridine and acetic anhydride.Measuring Gas chomatography-mass(GC-MS) fingerprints of water-soluble sugar,then determining the water-soluble components of 20 herbs by mass spectrometry and analyzing the results by Fisher method.Results A discriminant function had been established by Fisher.The Fisher discriminant analysis could distinguish the cold and heat properties about 20 herbs that the group back substitution is 100%.From the results,it confirmes that there is difference between the cold and heat properties in the GC-MS fingerprints by Fisher method,which shows that there are different water-soluble sugar in the cold and the heat herbs.Conclusion The Fisher analysis could differentiate the cold and heat properties of herbs,and it is an effective statistical tool to study the relationship between the cold and heat properties and water-soluble sugar.It concludes that the water-soluble sugar is one of the material basis of four properties of herbs.
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