扶正化瘀复方药物血清对大鼠肝贮脂细胞增殖及胶原合成的影响

Effect of Sera Containing Fuzheng Huayu(FZHY) Decoction on Ito Cell Proliferation and Collagen Synthesis in Rats

摘要:

采用正常大鼠灌胃给药、分离血清而体外作用培养细胞的血清药理学方法,探讨扶正化瘀方对培养大鼠贮脂细胞功能的影响。结果表明该复方血清无明显细胞毒性,抑制细胞增殖及胶原合成,而且该作用与复方的体内给药方式、给药剂量、取血时间、药物血清作用浓度等有一定关系。

Abstract:

Using a serum pharmacological method that the sera,derived from the rats orally taking FZHY,were incubated with culturd ito cells(Fat-storing cells,passage 1) of the rats,it wasinvestigated that FZHY affected ito cell functions such a cell proliferation and collagen synthesi.The results showed that the FZHY sera had no cytotoxicity towards the ito cells inhibited cell proliferation and collagen synthesis.Furthermore,this inhibition had a positive relation with administration and dosage of the FZHYdecoction bood-taken tine,and the concentration of the sera in the culture,was concluded that the inhibition of FZHY on ito cells may be one of mechanisms through which liver fibrosis was prevented with it.