树脂吸附-薄层扫描法测定抗病毒口服液菝葜皂甙元含量

Determination of Sarsasapogenin in an Anti-virus Oral Liquidwith a Resin-absorptive and TLCS Method

摘要:

利用抗病毒口服液中知母皂甙被大孔树脂吸附,除去糖等杂质干扰;再用75%乙醇洗脱皂甙。加酸水解生成皂甙元,经有机溶媒富集后,按薄层扫描法测定菝葜皂甙元含量。该法专一性强,灵敏度高,线性关系好,回收率为97.2%,RSD为3.28%。

Abstract:

The timosaponin in an anti-virus oral liquid was absorbed by great occipital resin to get rid of the disturbance of impurities like sugar,then eluted with 75% alcohol and hydrolyzed by acid to produce sapogenin,whose content was determined with a TCLS-method was sensitive with high specificity and good linear relationship,and the rate of recovery was 97.2%,RSD3.28%.