抗肝纤维化有效中药复方血清药理学方法探讨

Study on a Seropharmacological Method for an Effective Antifibrotic Formula

摘要:

扶正化瘀方灌胃正常大鼠制备血清，温育大鼠肝细胞及星状细胞，将含药血清及冻干粉直接添加于培养细胞，通过观察细胞形态及功能变化，探讨复方抗肝纤维化的血清药理试验方法。结果表明冻干粉抑制细胞增殖及活力。二次给药1h后同种灭活血清抑制星状细胞增殖、促进肝细胞增殖，能较好地用于抗肝纤维化的体外药理试验。

Abstract:

The serum was collected from rats fed on Fuzheng Huayu (FZHY) decoction, and FZHY decoction was lyophilized into powder. And both were incubated with hepatic stellate cells (HSC) and hepatocytes. Then cell morphology and functions were observed to study the seropharmacological methods for antifibrotic traditional Chinese herbs. The results showed that the FZHY powder inhibited cell proliferation and viability. The deactivated rat's sera drawn 1h after FZHY-treatment twice had no cytotoxity, promoted hepatocyte proliferation, and inhibited HSC proliferation. Therefore, this seropharmacological method could be applied for antifibrotic herbal formulae.