RP-HPLC测定补肾宁片中淫羊藿甙的含量

Determination of Icariin in Bushenning Tablet by RP-HPLC

摘要:

用反相高效液相色谱测定补肾宁片中淫羊藿甙含量?淫羊藿甙与其相邻峰的分离度是1.8;理论板数按淫羊藿甙峰计算是1697;对照品线性范围是4.88～78.02μg/ml,回归方程Y=0.00007187x+2.124,r=0.9998,平均回收率99.4%,RSD为0.48?此法操作简便,结果准确?

Abstract:

A quantitative method was developed for the determination of icariin in Bushenning tablet by reversed phase HPLC.The number of theoretical plates calculated by Icariin peak was 1697,R=1.8.The standard curve was linear in the concentration range of 4.88～78.02μg/ml,and correlation coefficient was 0.9998.The average recovery and the relative standard deviation were 99.4% and 0.48%,rectivily.