牛膝中齐墩果酸的提取工艺研究

Studies on the Extracting Technique of Oleanolic Acid from Achyranthes bidentata Bl

摘要:

目的:筛选牛膝中齐墩果酸的最佳提取工艺。方法:采用正交设计L₁₈(3⁷),用高效液相色谱法对不同提取工艺牛膝中的齐墩果酸的含量进行测定。结果:牛膝中齐墩果酸的最佳提取工艺为A₁B₂C₁D₁,即20目的药材,加入70%的乙醇水浴回流提取1h,只需一次。

Abstract:

Objective:To screening the optimized extracting technique of oleanolic acid from Achyranthes bidentats Bl.Methods:To determine the content of oleanolic acid with different extracting technique by the orthogonal designed methods(L₁₈(3⁷).Results:The optimum extracting technique is A₁B₂C₁D₁,that is the crude drug in twenty mesh refluxed by 70% alcohol for one hour.