黄连解毒汤含药血清对LPSTNF-α诱导的人中性粒细胞与血管内皮细胞间粘附的影响

Influence of Herbage Containing Serum of HuangLianJieDu Tang on LPS or TNF-α Induced Neutrophil-Endothelium Cells Adhesion

摘要:

以体外培养的人脐静脉内皮细胞(HUVEC)为模型,应用蛋白染料染色法,观察HLJDT含药血清及其与LPSINF-α共同处理HUVEC4h、12h、24h后,对中性粒细胞与血管内皮细胞粘附的影响。结果显示,HLJDT含药血清不仅能抑制非致炎状态下中性粒细胞与血管内皮细胞的粘附,而且能抑制致炎因子所诱导的中性粒细胞与血管内皮细胞粘附作用增强,这可能是黄连解毒汤抗炎作用的机制之一。

Abstract:

Objects:To investigate the influence of herbage containing serum of HuangLianJieDu Tang(HLJDT)on LPS or TNF-α induced neutrophil-endothelium adhesion and its anti-inflammation mechanism.Methods:Based on the model of Human umbilical vein endothelial cell(HUVEC),Rose Bengal Stain was used to study the influence of herbage containing serum of HLJDT on LPS or TNF-α induced neutrophil-endothelium adhesion by pretreated HUVEC4h、12h、24h.Results:The results showed the seram of 1.5h group can decrease cells adhesion by pretreated HUVEC with serum only,but all serum can decrease LPS/INF-α induced cells adhesion by pretreated HUVEC with serum and LPS or TNF-α.Conclusion:The herbage containing serum of HLJDT can decrease the neutrophil-endothelium cells adhesion,inhabiting neutrophil transude from vessel,which may be one of its anti-inflammation mechanisms.