益心康胶囊含药血清对氧应激所致线粒体损伤的保护作用

The Protective Effect of Yi-Xin-Kang Capsule-containing Serum on Mitochondria Injury Induced by Oxygen Stress

摘要:

目的和方法:应用羟自由基损伤体系(Fe²⁺/H₂O₂)在体外与大鼠心肌线粒体共同孵育30min,观察了氧应激对心肌线粒体的影响,探讨益心康胶囊(H303)含药血清对氧应激损伤的保护机制。结果:不同浓度Fe²⁺/H₂O₂可使反应体系中脂质过氧化产物明显升高;线粒体超微结构发生明显改变;线粒体肿胀度增加;线粒体GSH含量明显降低。H303含药血清可明显减轻上述损伤,表现为显著抑制线粒体脂质过氧化和GSH含量的降低;改善线粒体超微结构,减轻线粒体肿胀度。结论:氧应激可造成心肌线粒体结构和功能损伤。H303抗心肌缺血损伤的作用机制是通过防止线粒体脂质过氧化,保护内源性抗氧化物,改善线粒体结构等作用而实现的。

Abstract:

The effect of oxygen stress on myocardial mitochondria of rat and the protective effect of Yi-Xin-Kang capsule(H303)-containing serum on oxygen stress-induced injury by incubating myocardial mitochondria with hydroxy free radical generating system (Fe²⁺/H₂O₂) for 30 min,were observed.Result shows Fe²⁺/H₂O₃ induced obvious injury of mitochondria concentration-dependently.It was also found that mitochondria were significantly occurred with lipid peroxidation and swelled,the ultrastructures of mitochondria were changed and the content of GSH was markedly reduced.H303-containing serum showed significant protective effects on mitochondria injury induced by oxygen stress,and antagonized mitochondria lipid peroxidation and the decrease of endogenous GSH.The ultrastuctures of mitochondria and their swelling degree were also improved.