四物汤对环磷酰胺所致血虚证小鼠造血细胞作用的研究

Effects of Siwu Tang on Medullary Hematopoiesis in Mice of Blood Deficiency Induced by Cyclophosphamide

摘要:

探讨四物汤治疗环磷酰胺所致小鼠血虚证的作用机理。腹腔注射环磷酰胺制成小鼠血虚证模型;测定小鼠外周血象;骨髓细胞集落形成;CD34⁺细胞在骨髓有核细胞中的比例;小鼠胸腺指数。结果表明四物汤具有明显促进骨髓造血和增强免疫的作用,并可升高血虚证小鼠的外周血象。四物汤增强免疫、促进造血可能是四物汤治疗小鼠血虚证的机理之一。

Abstract:

To study the effects of Siwu Tang on the mice of blood deficiency induced by cyclophosphamide,we employed the methods of circulating blood cells detection by microcellcounter,flow cytometric enumeration of CD34⁺ himatopoietic stem and progenitor cells by double fluorescent labeling.The results showed Siwu Tang promoted the recovery of stem and progenitor cells and circulating WBC on the blood deficient mice,and enhanced immune function.proliferative effect of Siwu Tang on bone marrow may play a key role in improvement of hematopoiesis with Siwu Tang treating blood deficient mice.